A different sequencing method that chemically cleaved DNA at specific bases, developed by Allan Maxam and Walter Gilbert, was the dominant technology into the 1980s. Radiolabeled DNA samples were incubated in four separate reactions, each of which contained a chemical that cleaved after a different nucleotide — either A/G, G, C, or C/T. By adding the right amount of each chemical, it was possible to produce different fragments chopped off at each individual base. The sequence could then be read using gel electrophoresis and autoradiography. Maxam–Gilbert sequencing was easier than the plus and minus method to run and interpret, but was eventually surpassed by Sanger’s chain termination method, which molecular biologists found both technically preferable and more “elegant” since it mirrored the natural copying of DNA.
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。电影对此有专业解读
Маргарита Щигарева
Последние новости。业内人士推荐heLLoword翻译官方下载作为进阶阅读
Фото: Keystone Press Agency / Global Look Press
Российский актер Гоша Куценко рассказал, как встретил звезду Голливуда Брюса Уиллиса при курьезных обстоятельствах. Забавный случай артист вспомнил на шоу Иды Галич «Есть вопросики».，详情可参考币安_币安注册_币安下载